Publications 2011 – 2016

  1. Jakutyte-Giraitiene, L. & Gasiunas, G., Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1 Journal of Industrial Microbiology & Biotechnology, 2016, 1-6. (PDF)
  2. Kazlauskiene, M., Tamulaitis, G., Kostiuk, G., Venclovas, Č. & Siksnys, V., Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition. Mol Cell, 2016, vol. 62, 295-306. (PDF)
  3. Labrie, V., Buske, O. J., Oh, E., Jeremian, R., Ptak, C., Gasiūnas, G., Maleckas, A., Petereit, R., Žvirbliene, A., Adamonis, K., Kriukienė, E., Koncevičius, K., Gordevičius, J., Nair, A., Zhang, A., Ebrahimi, S., Oh, G., Šikšnys, V., Kupčinskas, L., Brudno, M. & Petronis, A., Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging. Nat Struct Mol Biol, 2016, vol. 23, 566-573. (PDF)
  4. Müller, M., Lee, C. M., Gasiunas, G., Davis, T. H., Cradick, T. J., Siksnys, V., Bao, G., Cathomen, T. & Mussolino, C., Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome. Mol Ther, 2016, vol. 24, 636-644. (PDF)
  5. Siksnys, V. & Gasiunas, G., Rewiring Cas9 to Target New PAM Sequences. Mol Cell, 2016, vol. 61, 793-794. (PDF)
  6. Glemzaite, M., Balciunaite, E., Karvelis, T., Gasiunas, G., Grusyte, M. M., Alzbutas, G., Jurcyte, A., Anderson, E. M., Maksimova, E., Smith, A. J., Lubys, A., Zaliauskiene, L. & Siksnys, V., Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex. RNA Biol, 2015, vol. 12, 1-4. (PDF)
  7. Karvelis, T., Gasiunas, G., Young, J., Bigelyte, G., Silanskas, A., Cigan, M. & Siksnys, V., Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements. Genome Biol, 2015, vol. 16, 253. (PDF)
  8. Rutkauskas, M., Sinkunas, T., Songailiene, I., Tikhomirova, M. S., Siksnys, V. & Seidel, R., Directional R-Loop Formation by the CRISPR-Cas Surveillance Complex Cascade Provides Efficient Off-Target Site Rejection. Cell Rep, 2015. (PDF)
  9. Sasnauskas, G., Zagorskaitė, E., Kauneckaitė, K., Tamulaitiene, G. & Siksnys, V., Structure-guided sequence specificity engineering of the modification-dependent restriction endonuclease LpnPI. Nucleic Acids Res, 2015, vol. 43, 6144-6155. (PDF)
  10. Sinkunas, T., Gasiunas, G. & Siksnys, V., Cas3 nuclease-helicase activity assays. Methods Mol Biol, 2015, vol. 1311, 277-291. (PDF)
  11. Tamulaitis, G., Rutkauskas, M., Zaremba, M., Grazulis, S., Tamulaitiene, G. & Siksnys, V., Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI. Nucleic Acids Res, 2015, vol. 43, 8100-8110. (PDF)
  12. Zaremba, M. & Siksnys, V., An Engineered SS Bridge Blocks the Conformational Change Required for the Nuclease Activity of BfiI. Biochemistry, 2015, vol. 54, 5340-5347. (PDF)
  13. Zaremba, M., Toliusis, P., Grigaitis, R., Manakova, E., Silanskas, A., Tamulaitiene, G., Szczelkun, M. D., Siksnys, V., Tamulaitiene, G., Silanskas, A., Grazulis, S., Zaremba, M. & Siksnys, V., DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis. Nucleic Acids Res, 2015, vol. 43, 3405. (PDF)
  14. Gasiunas, G., Sinkunas, T. & Siksnys, V., Molecular mechanisms of CRISPR-mediated microbial immunity. Cell Mol Life Sci, 2014, vol. 71, 449-465. (PDF)
  15. Golovenko, D., Manakova, E., Zakrys, L., Zaremba, M., Sasnauskas, G., Gražulis, S. & Siksnys, V., Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme. Nucleic Acids Res, 2014, vol. 42, 4113-4122. (PDF)
  16. Rutkauskas, D., Petkelyte, M., Naujalis, P., Sasnauskas, G., Tamulaitis, G., Zaremba, M. & Siksnys, V., Restriction enzyme Ecl18kI-induced DNA looping dynamics by single-molecule FRET. J Phys Chem B, 2014, vol. 118, 8575-8582. (PDF)
  17. Szczelkun, M. D., Tikhomirova, M. S., Sinkunas, T., Gasiunas, G., Karvelis, T., Pschera, P., Siksnys, V. & Seidel, R., Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes. Proc Natl Acad Sci U S A, 2014, vol. 111, 9798-9803. (PDF)
  18. Tamulaitiene, G., Silanskas, A., Grazulis, S., Zaremba, M. & Siksnys, V., Crystal structure of the R-protein of the multisubunit ATP-dependent restriction endonuclease NgoAVII. Nucleic Acids Res, 2014, vol. 42, 14022-14030. (PDF)
  19. Tamulaitis, G., Kazlauskiene, M., Manakova, E., Venclovas, Č., Nwokeoji, A. O., Dickman, M. J., Horvath, P. & Siksnys, V., Programmable RNA shredding by the type III-A CRISPR-Cas system of Streptococcus thermophilus. Mol Cell, 2014, vol. 56, 506-517. (PDF)
  20. Zagorskaitė, E. & Sasnauskas, G., Chemical Display of Pyrimidine Bases Flipped Out by Modification-Dependent Restriction Endonucleases of MspJI and PvuRts1I Families PLoS ONE, 2014, vol. 9, e114580. (PDF)
  21. Zaremba, M., Toliusis, P., Grigaitis, R., Manakova, E., Silanskas, A., Tamulaitiene, G., Szczelkun, M. D. & Siksnys, V., DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis. Nucleic Acids Res, 2014, vol. 42, 13887-13896. (PDF)
  22. Gasiunas, G. & Siksnys, V., RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing? Trends Microbiol., 2013, vol. 21, 562-7. ()
  23. Horvath, P., Gasiunas, G., Siksnys, V. & Barrangou, R., Applications of the Versatile CRISPR-Cas Systems , 2013, 267-286. (PDF)
  24. Karvelis, T., Gasiunas, G., Miksys, A., Barrangou, R., Horvath, P. & Siksnys, V., crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus RNA Biol., 2013, vol. 10, 841-51. ()
  25. Karvelis, T., Gasiunas, G. & Siksnys, V., Programmable DNA cleavage in vitro by Cas9 Biochem. Soc. Trans., 2013, vol. 41, 1401-6. ()
  26. Sinkunas, T., Gasiunas, G., Waghmare, S. P., Dickman, M. J., Barrangou, R., Horvath, P. & Siksnys, V., In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J, 2013. (PDF)
  27. Baranauskas, A., Paliksa, S., Alzbutas, G., Vaitkevicius, M., Lubiene, J., Letukiene, V., Burinskas, S., Sasnauskas, G. & Skirgaila, R., Generation and characterization of new highly thermostable and processive M-MuLV reverse transcriptase variants Protein Engineering Design and Selection, 2012, vol. 25, 657-668. (PDF)
  28. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V., Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A, 2012, vol. 109, E2579-E2586. (PDF)
  29. Manakova, E., Grazulis, S., Zaremba, M., Tamulaitiene, G., Golovenko, D. & Siksnys, V., Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease. Nucleic Acids Res, 2012, vol. 40, 6741-6751. (PDF)
  30. Silanskas, A., Zaremba, M., Sasnauskas, G. & Siksnys, V., Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates. Bioconjug Chem, 2012, vol. 23, 203-211. (PDF)
  31. Sukackaite, R., Grazulis, S., Tamulaitis, G. & Siksnys, V., The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine. Nucleic Acids Res, 2012, vol. 40, 7552-7562. (PDF)
  32. Zaremba, M., Sasnauskas, G. & Siksnys, V., The link between restriction endonuclease fidelity and oligomeric state: a study with Bse634I. FEBS Lett, 2012, vol. 586, 3324-3329. (PDF)
  33. Kostiuk, G., Sasnauskas, G., Tamulaitiene, G. & Siksnys, V., Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease. Nucleic Acids Res, 2011, vol. 39, 3744-3753. (PDF)
  34. Sapranauskas, R., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res, 2011, vol. 39, 9275-9282. (PDF)
  35. Sasnauskas, G., Kostiuk, G., Tamulaitis, G. & Siksnys, V., Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation. Nucleic Acids Res, 2011, vol. 39, 8844-8856. (PDF)
  36. Silanskas, A., Foss, M., Wende, W., Urbanke, C., Lagunavicius, A., Pingoud, A. & Siksnys, V., Photocaged variants of the MunI and PvuII restriction enzymes. Biochemistry, 2011, vol. 50, 2800-2807. (PDF)
  37. Sinkunas, T., Gasiunas, G., Fremaux, C., Barrangou, R., Horvath, P. & Siksnys, V., Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. EMBO J, 2011, vol. 30, 1335-1342. (PDF)